|
BASIC MODE
|
| To measure the Absorbance and Transmittance |
| |
|
QUANTITATIVE
|
|
1. Coefficient Method
|
|
2. Standard Method curve.
|
|
Up to 10 standard samples may be used to establish a curve. Four methods to fitting a curve through the calibration points: Linear fit through zero, square fit & cubic fit.
|
| |
|
WAVELENGTH SCAN
|
|
1. The wavelength scan intervals are 0.1, 0.2, 0.5, 1, 2 & 5 nm
|
|
2. Fast, Medium & Slow Scan speeds are available. They very from100 to 1800 nm/min
|
|
3. Wavelength are scanned from high to low so that the instrument waits at high Wavelength and minimizes the degradation of UV sensitive samples
|
| |
|
MULTI-WAVELENGTH
|
|
Up to 32 wavelengths can be selected and multiple samples can be measured
|
|
|
|
KINETICS
|
|
This mode may be used for time course scanning or reaction rate calculations. Abs. vs. time graphs is displayed on the screen in real time
|
|
Wait time and measurement time up to 12 hours may be entered with time intervals of 0.5,1,2,5,10,30 seconds & 1min. Post- run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor
|
| |
|
DNA/PROTEIN TEST
|
| Concentration and DNA purity are quickly and easily calculated: Absorbance ratio s 260nm/280nm with optional subtracted absorbance at 320nm. |
|
DNA concentration= 62.9x A260-36.0x A280
|
|
Protein concentration=1552xA260-757.3xA280
|
| |
|
UTILITY
|
|
Has following functions
|
|
WL Reset, Printer, Lamp, Clock, Dark Current, Accu. Validity, WL Validity Connect to PC, Beeper On/Off & System Base Line
|
|